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Beilstein J. Org. Chem. 2017, 13, 874–882, doi:10.3762/bjoc.13.88
Graphical Abstract
Figure 1: Expression of GFP-tagged GAT1 in infected insect cells. (A) Flow cytometry analysis of GAT1/GFP in ...
Figure 2: Protein fingerprinting. (A) Coomassie Blue staining of the GAT1/GFP protein from infected Sf9 cells...
Figure 3: Isolation of the GAT1/GFP fusion protein from Sf9 cells by mAb-GFP-conjugated affinity column chrom...
Figure 4: Purification of the GAT1/GFP fusion protein from Sf9 cells by SE-FPLC. (A) Elution profile of GAT1/...
Figure 5: Characterization of the GAT1/GFP fusion protein after SE-FPLC. (A) Elution profile of GAT1/GFP prot...
Beilstein J. Org. Chem. 2012, 8, 2067–2071, doi:10.3762/bjoc.8.232
Figure 1: Structures of two novel linkers on different resins.
Scheme 1: Synthesis of a new acylsulfonamide safety-catch linker. Reagents and conditions: (a) benzaldehyde, ...
Scheme 2: Functionalization of different resins. Reagents and conditions: (a) Cs2CO3, DMF, TBAI, Merrifield c...
Scheme 3: Glycosylation and cleavage reactions for analysis. Reagents and conditions: (a) automated glycosyla...
Scheme 4: Further investigations of safety-catch linker. Reagents and conditions: (a) NaOMe, MeOH; (b) 19, NI...